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John Hansen

Research Microbiologist

Contact Info


Short Biography

Education:

1995-2001: Postdoc, Basel Institute for Immunology, Basel, Switzerland.

1991-1995: Ph.D.  Genetics/Immunology, Dept. of Microbiology, Oregon State University.

1984-1988: B.S. in  Zoology & Chemistry, University of Wisconsin at Eau Claire.

Dr. Hansen is a Research Immunologist at the Western Fisheries Research Center. His laboratory utilizes a basic research, comparative approach to understand host-pathogen interactions with an emphasis on innate and cellular immunity.

Professional Experience:

Research Immunologist/Project Leader: USGS Western Fisheries Research Center, Aug. 2004-present.

Current projects:

1.  Development of zebrafish as an alternative model for assessing the impact of environmental chemicals on vertebrate health.

2.  Functional roles of the "inflammasome" during intracellular infection-focus on innate immunity

3.  US Veterinary Immune Reagent Network (www.umass.edu/vetimm/).

4.  Zebrafish and trout as models for understanding host-pathogen interactions in fish.

Affiliate Associate Professor: Global Health and Pathobiology, University of Washington, 2004-present with full graduate faculty status. http://bit.ly/nVTMQF  

Assistant Professor: Center of Marine Biotechnology, University of Maryland, 2001-2004. Functional genomics of antigen presentation, evolution of the immune system and development of cellular immunity in teleosts. http://bit.ly/dpQ0Gi

Adjunct Assistant Professor: Molecular Medicine, University of Maryland Baltimore. (2001-2004)

Member: Basel Institute for Immunology. Examined the organization, expression and functionality of the major histocompatibility locus (MHC) in teleosts and the generation of molecular and cellular reagents for examining the development and function of trout leukocytes. (1995-2001). http://bit.ly/dkpxSP

Graduate Research Assistant, Dept. of Microbiology (with Steve Kaattari), Oregon State University, 1991-1995. Ph.D. thesis research: Characterization of B-cell lymphopoiesis in rainbow trout.

Senior Laboratory Technician, Immunobiology Section-VA Hospital, Minneapolis, MN, 1988-1991. Coordinated the planning and execution of research projects investigating the role(s) that pro-inflammatory cytokines play in the pathogenesis of alcoholic liver disease.

 

 



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Last 10 years:

Current

Ray, K., Culcutt, M., Woodson, JC., and Hansen, J.D. Virulence factors encoded by the Francisella tularensis pathogenicity island are functionally conserved in the fish pathogen Francisella noatunensis. In preparation.

Saha NR, Ota T, Litman GW, Hansen, J, Parra Z, Hsu E, Buonocore F, Canapa A, Cheng JF, Amemiya CT. Genome complexity in coelacanth is reflected in its adaptive immune system. Journal of Experimental Zoology-Molecular and Developmental Evolution: http://www.ncbi.nlm.nih.gov/pubmed/24464682

Boudinot, P., Zou, J., Ota, T., Buonocore, F., Scapigliati, G., Canapa, A., Cannon, J., Litman, G., and J.D. Hansen. A tetrapod-like repertoire of innate immune receptors and effectos for Latimeria: an emphasis on antiviral immunity. Journal of Experimental Zoology-Molecular and Developmental Evolution: http://www.ncbi.nlm.nih.gov/pubmed/24482296

2013

Phillips RB, Dekoning JJ, Brunelli JP, Faber-Hammond JJ, Hansen JD, Christensen KA, Renn SC, Thorgaard GH. Characterization of the Omy1 region on the trout Y chromosome. (2012). International J Genomics. ID 261730

2012

Vojtech, L.V., Scharping, N., Woodson, J.D. and J.D. Hansen. Roles of inflammatory caspases during processing of  zebrafish interleukin-1b in Francisella noatunensis infection. 2012 Infection and Immunity 80:2878-85.

Lefebvre, K.A., Frame, E.R., Gulland, F., Hansen, J.D., Kendrick, P.S., Beyer, R.P., Bammler, T.K., Farin, F.M., Hiolski, E.M., Smith, D.R., and D.J. Marcinek. (2012) A novel antibody-based biomarker for chronic algal toxin expsoure and sub-acute neurotoxicity. Plos One 7:e36213 www.ncbi.nlm.nih.gov/pmc/articles/PMC3342169/?tool=pubmed

Hansen, J.D., Woodson, J.D., Hershberger, P.K., Grady, C., Gregg, J.L. and M.K. Purcell. (2012) Induction of anti-viral genes during acute infection with Viral hemorrhagic septicemia virus (VHSV) genogroup IVa in Pacific herring (Clupea pallasii). Fish and Shellfish Immunology 32: 259-267.

Gomez-Ramirez, F., Green, W., Rego, K., Hansen, J.D., Costa, G., Kataria, P and E.S. Bromage. (2012) Discovery and characterization of secretory IgD in rainbow trout: sIgD is produced through a novel splicing mechanism.  Journal of Immunology 188: 1341-1349.

2011

Laing, K.J., and Hansen, J.D. Fish T cells: recent advances through genomics. Developmental and Comparative Immunology. 35: 1282-95.

Hansen, J.D., Vojtech, L., and Laing, K.J. Sensing disease and danger: a survey of vertebrate PRRs and their origins. Developmental and Comparative Immunology 35 (9): 886-897.

Hansen, J.D., Farrugia, T.J., Woodson, J.C., and Laing, K.J. Description of an elasmobranch TCR coreceptor: CD8a from Rhinobatos productus. Developmental and Comparative Immunology 35: 452-460.

Purcell, M.K., Mercy, I.S., Kurath, G., and Hansen, J.D. Microarray analysis of rainbow trout infected with two strains of Infectious hematopoietic necrosis virus that differ in virulence. Fish and Shellfish Immunology: 30: 84-93.

2009

Vojtech, L.N., Sanders, G.E., Conway, C., Ostland, V. and Hansen, J.D. Host immune response and acute disease in a zebrafish model of Francisella pathogenesis. Infection & Immunity 77:914-25.

Purcell, M.K., Laing, K.J., Woodson, J.C, Thorgaard, G.H. and Hansen, J.D. Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes.  Fish Shellfish Immunology 26:293-30.

Hansen, J.D., Du Pasquier, L., Lefranc, MP., Lopez, V., Benmansour, A and  Boudinot, P. The B7 family of immunoregulatory receptors: a comparative and evolutionary perspective. Molecular Immunology 46:457-72.

2008

Landis, E. L. Purcell, M., Thorgaard, G. and Hansen, J.D. Transcriptional profiling of MHC class I genes in rainbow trout infected with IHNV. Molecular Immunology 45: 1646-1657.

Laing, K., Purcell, M., Winton, J. and Hansen, J.D. A genomic view of the NOD like receptor family in teleost fish: identification of a novel subfamily in zebrafish. BMC Evolutionary Biology 8:42-57.

2007

Laing, K., Dutton, S., and Hansen, J.D. Molecular and biochemical analysis of rainbow trout LCK suggests a conserved mechanism for T-cell signaling in gnathostomes. Molecular Immunology 44:2737-48. 

Palti, Y., Rodriquez, M.F., Gahr, S.A. and Hansen, J.D. Evolutionary history of the ABCB2 genomic region in teleosts. Developmental and Comparative Immunology 31:483-98.

Bernard, D., Hansen, J.D., Du Pasquier, L., LeFranc, ML., Benmansour, A., and Boudinot, P. Costimulatory receptors in jawed vertebrates: conserverd CD28, odd CTLA4 and multiple BTLAs. Developmental and Comparative Immunology 31:255-71.

2006

Laing, K., Zhou, J., Purcell, M.K., Phillips, R., Secombes., C.J. and Hansen, J.D. Evolution of the CD4 family: teleost fish possess two divergent forms of CD4 in addition to LAG-3. Journal of Immunology 177: 3939-3951.

Purcell, M.K., K.M. Nichols, J.R. Winton, G.K. Kurath, G.H. Thorgaard, P. Wheeler, J.D. Hansen, R.P. Herwig and L.K. Park. Comprehensive gene expression profiling following DNA vaccination against infectious hematopoietic necrosis virus. Molecular Immunology: 43:2089-2106.

Landis, E.L., Palti, Y., DeKoning, J., Drew, R, Phillips, R. and Hansen, J.D. Identification and regulatory analysis of rainbow trout tapasin and tapasin-related  genes. Immunogenetics 58: 56-69.

Bernard, D., Riteau, B., Hansen, J.D., Phillips, R., Boudinot, P., and Benmansour, A. Costimulatory receptors in a teleost fish: typical CD28, elusive CTLA4. Journal of Immunology 176: 4191-2000.

2005

Hansen JD, Landis ED, Phillips RB. Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: Implications for a distinctive B cell developmental pathway in teleost fish. Proc. Natl. Acad. Sci. (PNAS) U S A. 102:6919-24.

H. Boshra.,Wang, T., Hove-Madsen, L., Hansen, J.D., Li, J., Matlapudi, A., Secombes, C., Tort, L., and Sunyer, J.O. Characterization of a C3a receptor in rainbow trout and Xenopus: The first identification of a C3a receptor in a non-mammalian species. Journal of Immunology 175:2427-37.

2004

Köllner, B., Fischer, U., Rombout, J.H.W.M., Taverne-Thiele, J.J. and Hansen, J.D.  Potential involvement of rainbow trout thrombocytes in immune functions: A study using a panel of monoclonal antibodies and RT-PCR. Developmental and Comparative Immunology 28:1049-62.

Ohta, Y., Boulay,T., Phillips, R., Flajnik, M.F. and Hansen, J.D.  Orthologs of CD83: isolation and characterization of a putative dendritic cell marker from elasmobranch and teleost fish. Journal of Immunology 173:4553-60.

Boshra, H., Li, J., Peters, R., Hansen, J.D., Matlapudi, A, and Sunyer, J.O.  Cloning, expression, cellular distribution and role in chemotaxis of a C5a receptor in rainbow trout: The first identification of a C5aR in a non-mammalian species. Journal of Immunology 172:4381-90.

Palti, Y., Gahr, S.A, Hansen, J.D and Rexroad , C.E. Characterization of a new BAC library for rainbow trout: evidence for multi-locus duplication.  Animal Genetics.  35:130-3.

Landis, E.L. and Hansen, J.D.  Annotation of two tapasin promoters from rainbow trout.  Marine Biotechnolgy 6: 112-118.

2003

Cupit, P.M*., Hansen, J.D.*., McCarty, A.S., White, G., Chioda, M., Spada, F., Smale, S.T, and Cunningham, C.C. Ikaros family members from the agnathan Myxine glutinosa and the urochordate Oikopleura dioica: emergence of an essential transcription factor for adaptive immunity. Journal of Immunology 171: 6006-6013.

Phillips, R., Zimmerman, A., Noakes, M., Palti, Y., Morasch, M., Ristow, S., Thorgaard, G.H. and Hansen, J.D.  Physical and genetic mapping of the rainbow trout major histocompatibility regions: evidence for duplication of the class I region.  Immunogenetics  55:561-569.




                           

My Science Topics


Science Topic
Subtopic
Environmental Issueshealth and disease
Environmental Issueshuman impacts
Plants and Animalsanimals
Plants and Animalsvertebrates



My USGS Science Strategy Areas

The Role of Environment and Wildlife in Human Health

Francisella mediated pathogenesis in zebrafish

Image of Current Focus for Francisella mediated pathogenesis in zebrafish

Presence of necrotizing granulomas in chronically infected zebrafish


Francisella noatunensis expressing GFP

Image of Francisella noatunensis expressing GFP

Strains of F. noutunensis, an emerging fish pathogen, were transformed with a construct that expresses GFP driven by a promoter from a Francisella "house keeping" gene. GFP bacteria (left) and non-transformed bacteria as visualized by a blue-light emitting diode. These fluorescent strains can be used to visulize infection in vivo.


Contact Information

John Hansen
6505 N.E. 65th Street
Seattle, WA 98115
jhansen@usgs.gov
206-526-6588
206-526-6654 - Fax
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