USGS Professional Pages
Environmental and Public Health MicrobiologistContact Info
Dale received a B.S. in Microbiology in 1990 from the Department of Biology, University of South Florida (USF). In December of 1994 he received a Master of Science in Public Health, with a research focus on methods development (IMS-multiplex-PCR, dot blot) for the detection of two pathogenic protozoa (Cryptosporidium parvum and Giardia lamblia) in environmental samples, from the College of Public Health, USF. In December of 1999 he received a Doctorate of Philosophy with a research focus on the use of molecular methods for detection of water quality indicator microorganisms and pathogenic viruses in fresh and marine waters from the Department of Marine Sciences, USF, St. Petersburg, Florida (major advisor, Dr. Joan B. Rose). In his first Post Doctoral position at USF’s College of Marine Science, he worked with Dr. John H. Paul III on human enterovirus detection assays, marine lysogeny and isolation of viruses lytic to the red-tide agent Karenia brevis. In his second Post Doctoral position at the United States Geological Survey in St. Petersburg Florida, he worked with Dr. Eugene A. Shinn on a NASA funded grant to study microbiology and public health issues associated with atmospheric transatlantic transport of African dust to the Caribbean and Americas. Dale is currently employed by the U.S. Geological Survey as an environmental public health microbiologist and is working on microbial water quality issues and long range dust storm associated dispersion of microorganisms around the globe.
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Publications (Peer Review)
1. Johnson, D.W., N.J. Pieniazek, D.W. Griffin, L. Misener, and J.B. Rose. 1995. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Applied and Environmental Microbiology. 61:3849-3855
2. Paul, J.H., J.B. Rose, S.C. Jiang, X. Zhou, P. Cochran, C. Kellogg, J.B. Kang, D.W. Griffin, S. Farrah and J. Lukasik. 1997. Evidence for ground water and surface water contamination by wastewater disposal wells in the Florida Keys. Water Research. 31(6):1448-1454
3. Rose, J.B., X. Zhou, D.W. Griffin, and J.H. Paul. 1997. Comparison of PCR and Plaque assay for detection and enumeration of coliphage in polluted marine waters. Applied and Environmental Microbiology. 63(11): 4564-4566
4. Griffin, D.W., C.J. Gibson III, E.K. Lipp, K. Riley, J.H. Paul and J.B. Rose. 1999. Detection of viral pathogens by RT-PCR and of microbial indicators using standard methods in the canals of the Florida Keys. Applied and Environmental Microbiology. 65(9):4118-4125
5. Griffin, D.W., R. Stokes, J.B. Rose, and J.H. Paul. 2000. Bacterial indicator occurrence and the use of an F+ specific RNA coliphage assay to identify fecal sources in Homosassa Springs, Florida. Microbial Ecology. 39:56-64
6. Paul, J.H., A. Alfreider, J.B. Kang, R.A. Stokes, D. Griffin, L. Cambell and E. Ornolfsdottir. 2000. Form IA rbcL Transcripts Associated with a Low Salinity/High Chlorophyll Plume ("Green River") in the Eastern Gulf of Mexico. Marine Ecology Progress Series. 198:1-8
7. Paul, J.H., M.R. McLaughlin, D. Griffin, E.K. Lipp, R. Stokes, and J.B. Rose. 2000. Rapid Movement of Wastewater from Onsite Disposal Systems into Surface Waters in the Lower Florida Keys. Estuaries. 23(5):662-668
8. Rose, J.B., D.W. Griffin and L.W. Nicosia. 2000. Virus Transport – From Septic Tanks to Coastal Waters. Small Flow Quarterly. 1(3):20-23
9. Griffin, D.W., V.H. Garrison, J.R. Herman and E.A. Shinn. 2001. African Desert Dust in the Caribbean Atmosphere: Microbiology and Public Health. Aerobiologia. 17(3):203-213
10. Solicited article - Griffin, D.W., C.A. Kellogg, and E.A. Shinn. 2001. Dust in the Wind: Long Range Transport of Dust in the Atmosphere and its Implications for Global Public and Ecosystem Health. Global Change and Human Health. 2(1):20-33
11. Griffin, D.W., E.K. Lipp and J.B. Rose. 2001. Marine Recreation and Public Health Microbiology: Quest for the Ideal Indicator. BioScience. 51(10):817-825
12. McDaniel, L., D.W. Griffin, J. Crespo-Gomez, M.R. McLaughlin and J.H. Paul. 2001. Evaluation of marine bacterial lysogens for development of a marine prophage induction assay. Marine Biotechnology. 3:528-535
13. Paul, J.P., L. Houchin, D. Griffin, T. Slifko, M. Guo, B. Richardson, and K. Steidinger. 2002. A filterable lytic agent obtained from a Red Tide bloom that caused lysis of Karenia brevis (Gymnodinum breve) Cultures. Aquatic Microbial Ecology. 27(1):21-27
14. Griffin, D.W., C.A. Kellogg, K.K. Peak and E.A. Shinn. 2002. A Rapid and Efficient Assay for Extracting DNA from Fungi. Letters in Applied Microbiology. 34(3):210-214
15. Solicited article – Griffin, D.W. 2002. Drinking Water, Microbiology and Home Defense. Water Conditioning and Purification. 44(4):34-37
16. Solicited article - Griffin, D.W., C.A. Kellogg, V.H. Garrison and E.A. Shinn. 2002. The Global Transport of Dust: An intercontinental river of dust, microorganisms and toxic chemicals flows through the Earth’s atmosphere. American Scientist. 90(3):228-235
17. Lipp, E.K., J.L. Jarrell, D.W. Griffin, J. Lukasik, J. Jacukiewicz and J.B. Rose. 2002. Preliminary evidence of human fecal contamination in corals of the Florida Keys. Marine Pollution Bulletin. 44(7):666-670
18. Donaldson, K.A., D.W. Griffin and J.H. Paul. 2002. Detection, quantitation and identification of enteroviruses from surface waters of the Florida Keys using Real-Time RT-PCR. Water Research. 36(10):2505-2514
19. Solicited article - Griffin, D.W., K.A. Donaldson, J.H. Paul, and J.B. Rose. 2003. Pathogenic Human Viruses in Coastal Waters. Clinical Microbiology Reviews. 16(1):129-143
20. Garrison, V.H., E.A. Shinn, W.T. Foreman, D.W. Griffin, C.W. Holmes, C.A. Kellogg, M.S. Majewski, L.L. Richardson, K.B. Ritchie, and G.W. Smith. 2003. African and Asian dust: From desert soils to coral reefs. BioScience. 53(5):469-480
21. Wawrik, B., J.H. Paul, L. Campbell, D. Griffin, L. Houchin, A. Fuentes-Ortega and F. Muller-Karger. 2003. Vertical Structure of the Phytoplankton Community associated with a Coastal Plume in the Gulf of Mexico. Marine Ecology Progress Series. 251:87-101
22. Grasby, S.E., C.C. Allen, T.G. Longazo, J.T. Lisle, D.W. Griffin, and B. Beauchamp. 2003. Supraglacial Sulfur Springs and Associated biological Activity in the Canadian High Arctic – Signs of Life Beneath the Ice. Astrobiology. 3(3):583-596
23. Grasby, S.E., C.C. Allen, T.G. Longazo, J.T. Lisle, D.W. Griffin, and B. Beauchamp. 2003. Biogeochemical sulphur cycle in an extreme environment – life beneath a high arctic glacier, Nunavut, Canada. Journal of GeoChemical Exploration. 78-79:71-74
24. Griffin, D.W., C.A. Kellogg, V.H. Garrison, J.T. Lisle, T.C. Borden and E.A. Shinn. 2003. Atmospheric microbiology in the northern Caribbean during African dust. Aerobiologia. 19(3-4):143-157
25. Jarrell, J.L., E.K. Lipp, D.W. Griffin, J. Lukasik, T. Scott, D. Wait, M. Sobsey and J.B. Rose. 2004. Presence, Infectivity and Stability of Enteric Viruses in Water: Relationships to Marine Waters Quality in the Florida Keys. Marine Pollution Bulletin. 48(7-8):698-704
26. Kellogg, C.A., D.W. Griffin, V.H. Garrison, K.K. Peak, N. Royall, R.R. Smith, and E.A. Shinn. 2004. Characterization of Aerosolized Bacteria and Fungi from Desert Dust Events in Mali, West Africa. Aerobiologia. 20(2):99-110
27. Griffin, D.W. 2004. Terrestrial microorganisms at 20,000 meters in Earth’s atmosphere. Aerobiologia. 20(2):135-140
28. Solicited article – Shinn, E.A., D.W. Griffin, and D.B. Seba. 2004. Atmospheric transport of mold spores in clouds of desert dust. Archives of Environmental Health. 58(8):498-504
29. Solicited article – Griffin, D.W., and C.A. Kellogg. 2004. Dust storms and their impact on ocean and human health. EcoHealth. 1:284-295
30. Solicited article – Lipp, E.K. and D.W. Griffin. 2004. Analysis of Coral Mucus as an Improved Media for Detection of Enteric Microbes and Patterns of Sewage Contamination in Reef Environments. EcoHealth. 1:317-323
31. Kelemen, P., Kikawa, E., Miller, D.J., Abe, N., Bach, W., Carlson, R.L., Casey, J.F., Chambers, L.M., Cheadel, M., Cipriani, A., Dick, H.J.B., Faul, U., Garces, M., Garrido, C., Gee, J.S., Godard, M.M., Graham, D.W., Griffin, D.W., Harvey, J., Ildefonse, Bl, Iturrino, G.J., Josef, J., Meurer, W.P., Mrozewski, S., Paulick, H., Rosner, M., Schroeder, T., Seyler, M., Takazawa, W., and Ocean Drilling Program Leg 209 drills into mantle peridotite along the Mid-Atlantic Ridge from 14oN to 16oN. JOIDES Journal 30(1):14-19.
32. Fong, T.-T., D. Griffin, and E.K. Lipp. 2005. Molecular assays for targeting human and bovine enteric viruses in coastal waters and application for library independent source tracking. Applied and Environmental Microbiology. 71(4):2070-2078.
33. Griffin, D.W. 2005. Clouds of desert dust and microbiology: A mechanism of global dispersion. Microbiology Today. 32(Nov05):180-182.
34. Griffin, D.W., D.L. Westphal, and M.A. Gray. 2006. Airborne microorganisms in the African desert dust corridor over the mid-Atlantic ridge, Ocean Drilling Program, Leg 209. Aerobiologia. 22(3):211-226.
35. Solicited article – Kellogg, C.A., and D.W. Griffin. 2006. Aerobiology and the global transport of desert dust. Trends in Ecology and Evolution. 21(11):638-644.
36. Garrison, V.H., Carr, R.S., Foreman, W.T., Genualds, S., Griffin, D.W., Kellogg, C.A., Majewski, M.S., Mohammed, A., Nipper, M., Shinn, E.A., Simonich, S.L., and Smith, G.W. 2006 Saharan dust – a carrier of persistent organic pollutants, metals, and microbes to the Caribbean? Revista Biologia Tropical. 54(Suppl.3):9-21.
37. Griffin, D.W., N. Kubilay, M. Kocak, M.A. Gray, T.C. Borden, and E.A. Shinn. 2007. Airborne desert dust and aeromicrobiology over the Turkish Mediterranean coastline. Atmospheric Environment. 41:4050-4062.
38. Solicited article – Griffin, D.W. 2007. Atmospheric movement of microorganisms in clouds of desert dust and implications for human health. Clinical Microbiology Reviews. 20(3):459-477.
39. Lipp, E.K., J.C. Futch, and D.W. Griffin. 2007. Analysis of multiple enteric viral targets as sewage markers in coral reefs Marine Pollution Bulletin. 54:1897-1902.
40. Katz, B., and D. Griffin. 2008. Using chemical and microbiological indicators to track the impact from the land application of treated municipal wastewater and other sources on groundwater quality in a karstic springs basin. Environmental Geology. 55:801-821.
41. Griffin, D.W. 2008. Non-spore forming eubacteria at an altitude of 20,000 meters in Earth’s atmosphere: Extended incubation periods needed for culture-based assays. Aerobiologia. 24(1):19-25.
42. Katz, B.G., J.H. Davis, and D.W. Griffin. 2009. Groundwater quality impacts from the land application of treated municipal wastewater in a large karstic spring basin: Chemical and microbiological indicators. Science of the Total Environment. 407:2872-2886
43. Symonds, E.M., D.W. Griffin, M. Breitbart. 2009. Eukaryotic viruses in wastewater samples from the United States. Applied and Environmental Microbiology. 75(5):1402-1409.
44. Lyles, M.B., H.B. Fredrickson, A.J. Bednar, H.B. Fannin, D. Griffin, T.M. Sobecki. 2009. Medical Geology: Dust exposure and potential health risks in the Middle East. Geochimica Et Cosmochimica Acta. 72(12, A576):1-4
45. Solicited article - Griffin, D.W., V. Luna, T. Petrosky, and S.A. Morman. 2009. A Bacillus anthracis survey of North American soil using several long-range transects, a group of post-Katrina New Orleans soil samples, and multiplex-PCR. Applied Geochemistry. 24:1464-1471.
46. Smith, D.J., D.W. Griffin, and A.C. Schuerger. 2010. Stratospheric microbiology at 20km over the Pacific Ocean. Aerobiologia. 26(1):35-46.
47. Futch, J.C., D.W. Griffin, and E.K. Lipp. 2010. Human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the Upper Florida Keys. Environmental Microbiology. 12(4):964-974.
48. Katz, B.G., D.W. Griffin, P.B. McMahon, H.S. Harden, E. Wade, R.W. Hicks, and J.P. Chanton. 2010. Fate of effluent-borne contaminants beneath septic tank drainfields overlying a karst aquifer. Journal of Environmental Quality. 39:1181-1195.
49. Griffin, D.W., C. Gonzalez, N. Teigell, T. Petrosky, M. Lyles and D. Northup. 2010. Observations on the use of membrane filtration and liquid impingement to collect airborne-microorganisms in various atmospheric environments. Aerobiologia. 27(1):25-35.
50. Smith, D.J., D.W. Griffin, R.D. McPeters, P.D. Ward, and A.C. Schuerger. 2011. Microbial survival in the stratosphere and implications for global dispersal. Aerobiologia. 27(4):319-332.
51. Smith, D.J., D.W. Griffin and D. Jaffe. 2011. The high life: Movement of microbes through the atmosphere. EOS. 92(30):249-256.
52. Futch, J.C., D.W. Griffin, K. Banks, and E.K. Lipp. 2011. Evaluation of sewage source and fate on southeast Florida coastal reefs. Marine Pollution Bulletin. 62:2308-2316.
53. Smith, D.J., D.A. Jaffe, M.N. Birmele, D.W. Griffin, A.C. Schuerger, J. Hee and M.S. Roberts. 2012. Free tropospheric transport of microorganisms from Asia to North America. Microbial Ecology. 64(4):973-985.
54. Gonzalez-Martin, C., N. Teigell-Perez, M. Lyles, B. Valladares and D.W. Griffin. 2013. Understanding the global dispersion of microbes by dust storms: Epifluorescent direct counts of bacteria and viruses from various desert regions. Research in Microbiology. 164(1):17-21.
55. Jason R. Vogel, J.R., J. Lu, D. Griffin, H. Ip, M. Moser, H. Ryu, N. Ashbolt and J.S. Domingo. 2013. Impacts of migratory Sandhill Cranes (Grus Canadensis) on microbial water quality in the Central Platte River, Nebraska, USA. Water, Air, and Soil Pollution. 224:1576, 10.1007/s11270-013-1576-3.
56. Solicited - Griffin, D.W. 2013. The quest for extraterrestrial life: What about the viruses? Astrobiology. 13(8):774-783.
57. Smith, D., J. Timonen, D. Jaffe, D. Griffin, M. Birmele and M. Roberts. 2013. Intercontinental dispersal of bacteria and archeae by transpacific winds. Applied and Environmental Microbiology. 79(4):1134-1139.
58. Smith, D.J., and D.W. Griffin. 2013. Letter to the editor of PNAS reference ‘Deleon-Rodriguez et al., 2013 publication online before print, January 28, 2013 – Microbiome of the upper troposphere: Species composition and prevalence, effects of tropical storms, and atmospheric implications.’ Proceedings of the National Academy of Sciences. 110(23):E2084.
59. Griffin, D.W., M.A. Gray, M.B. Lyles and D.W. Northup. 2013. The transport of non-indigenous microorganisms into caves by human visitation: A case study at Carlsbad Caverns National Park. Geomicrobiology. 31(3):175-185.
60. Griffin, D.W., E. Silvestri, C.Y. Bowling, T. Boe, D.B. Smith and T.L. Nichols. 2014. Anthrax and the geochemistry of soils in the contiguous United States. Geosciences. 4(3):114-127.
61. Joyner, J., J.C. Futch, D.W. Griffin and E.K. Lipp. 2014. Analyses of fecal contamination sources in a remote tropical system: The Dry Tortugas National Park, FL. EcoHealth. Submitted.
62. Westrich, J.R., D.W. Griffin and E.K. Lipp. 2014. Vibrio bacteria growth response to Saharan dust nutrients. Proceedings of the National Academy of Sciences. Submitted.
63. Gonzalez-Martin, C., and D.W. Griffin. 2014. Analyses of different membrane filter based methods for use in aerobiology studies. Letters in Applied Microbiology. Submitted.
64. Teigell-Perez, N., C. Gonzalez-Martin, B. Valladares, D.J. Smith, and D.W. Griffin. 2014. The atmospheric dispersion of prophage. Research in Microbiology. Submitted.
65. Kolby, J.E., S.D. Ramirez, L. Berger, D. Griffin, M. Jocque and L.F. Skerratt. 2014. Atmospheric dispersal of amphibian chytrid fungus increases the global threat of chytridiomycosis. Aerobiologia. Submitted.
66. Silvestri, E.E., S. Perkins, R. Lordo, W. Kovacik, T. Nichols, C.Y. Bowling, D. Griffin and F.W. Schaefer, III. 2014. Assessing the potential for Bacillus spore transport from an indoor release to external environments. Biosecurity and Bioterrorism. Submitted.
67. Doyle, E., A. Biales, M. Focazio, D. Griffin, and K. Loftin. 2014. Effect-based screening methods for water quality characterization will augment conventional analyte-by-analyte chemical methods in research as well as regulatory monitoring. Environmental Science and Technology. Accepted/In Press.
1. Solicited - Griffin, D.W. 2001. Microbial Public Health Indicators in the Marine Environment. In Methods in Microbiology, Volume 30: Marine Microbiology, Editor, J.H. Paul, Academic Press, New York, NY. ISBN 0-12-521530-4: 541-558.
2. Solicited - Griffin, D.W. 2009. Desert dust storm microbiology: Issues in planetary health. In McGraw-Hill Yearbook of Science and Technology: 73-75.
3. Griffin, D.W. 2009. Airborne Desert Dust: Implications for Global Health. In International Seminar on Nuclear War and Planetary Emergencies, 40th Session, Erice, Italy, August 19th – 24th, 2009. Editor R. Ragaini, World Scientific Publishing Co. 219-222.
4. Griffin, D.W. 2010. Climate change and climate systems influence and control the atmospheric dispersion of desert dust: Implications for human health. In International Seminar on Nuclear War and Planetary Emergencies, 42th Session, Erice, Italy, August 19th – 24th, 2010. Editor R. Ragaini, World Scientific Publishing Co. 503-507.
5. Solicited – Griffin, D.W. 2012. Pathogenic human viruses in residential canals of the Florida Keys. In USEPA, South Florida Marine Environments: An ecological synthesis. University of Maryland IAN Press, Cambridge, MD. ISBN 978-0-982-2305-3-4.
6. Solicited - Lipp, E.K., D.W. Griffin, J.C. Futch. 2012. In-ground disposal of human sewage can contaminate nearshore waters and reefs with bacteria and viruses. In USEPA, South Florida Marine Environments: An ecological synthesis. University of Maryland IAN Press, Cambridge, MD. ISBN 978-0-982-2305-3-4.
7. Solicited – Futch, J.C., D.W. Griffin, E.K. Lipp. 2012. Navigational inlets act as conduits for land-based sources of pollution to the marine environment and reefs of South Florida. In USEPA, South Florida Marine Environments: An ecological synthesis. University of Maryland IAN Press, Cambridge, MD. ISBN 978-0-982-2305-3-4.
8. Solicited – Griffin, D.W. and E.N. Naumova with; J.C. McEntee, D. Castronovo, J.L. Durant, M.B. Lyles, F.S. Faruque, and D.J. Lary. 2012. Remote sensing applications for assessing the effects of air quality on human health: An emphasis on volcanic ash and desert dust. In Environmental Tracking for Public Health Surveillance. Editors S.A. Morain and A.M. Budge. CRC Press. ISBN-10: 041558471X, ISBN-13: 978-0415584715, Chapter 4:129-186.
9. Solicited - Gonzalez-Martin, C. and D.W. Griffin. 2014. The global dispersion of pathogenic microorganisms by dust storms and its relevance to agriculture. In Advances in Agronomy. Editor D.L. Sparks. Academic Press, ELSEVIER. ISBN 978-0-12-800131-8, Chapter 1:1-41.
Foreign reprint of articles
1. Griffin, D.W., C.A. Kellogg, V.H. Garrison, and E.A. Shinn. 2003. Il trasporto globale delle polveri. Le Scienze. 417:72-80(original publication - Griffin et al. 2002, American Scientist. 90(3):228-235)
2. Griffin, D.W., C. Kellogg, V. Garrison, E. Shinn. 2003. La mondialisation des poussieres. Pour La Science – Climatologie. 309. (original publication - Griffin et al. 2002, American Scientist. 90(3):228-235)
1. Griffin, D.W., D.E. Friedman, and J.B. Rose. 1994. What is Cryptosporidium. Convention Paper. Water Quality Association. Phoenix, Arizona.
2. Griffin, D.W. and J.B. Rose. 1995. The use of Magnetic Antibody Capture to enhance PCR Detection of Cryptosporidium oocysts in Environmental Water Samples. Proceedings, W.Q.T.C., New Orleans, Louisiana.
3. Paul, J.H., J.B. Rose and D.W. Griffin. 1998. Microbiological Testing of Crystal Springs, Pasco County, Florida. Final report for the Ambient Ground-Water Quality Monitoring Program of Southwest Florida Water Management District.
4. Griffin, D.W., R. Stokes, J.B. Rose and J.H. Paul. 1999. Microbiological Water Quality in the Homosassa Springs State Wildlife Park: A Water Quality Improvement Project (WQIP). Final Report for a Florida Department of Environmental Protection funded project.
5. Brown, M.T., M.D. Annable, J.J. Delfino, J.W. Jawitz, M. Cohen, E. Hall, J. Chanton, W. Burnett, H. Harden, J.B. Rose, J.H. Paul, E.K. Lipp and D.W. Griffin. 2000. Determination of an Appropriate Onsite Sewage System Setback Distance to Seasonally Inundated Areas. Report for a Florida Department of Health funded project.
6. Grimes, D.J., J.B. Rose, C.N. Hass, P. Payment, A.K. Bej, W. Burkhardt, J. Burkholder, R. Burlage, A.K. Camper, R.R. Colwell, J.A. Daly, R. DeLeon, T. Ford, A.J. Gil, M.J. Gilchrist, D.W. Griffin, A. Huq, W. Koster, A. Lammerding, B.H. Olson, J.H. Paul, T. Phillips, E. Russek-Cohen, G.S. Sayler, M.L. Tamplin, P. Teunis, C. Colgan, A. Lohse and E. Houston. 2001. Reevaluation of Microbial Water Quality: Powerful New Tools for Detection and Risk Assessment. A Report from the American Academy of Microbiology.
7. Griffin, D.W. and E.A. Shinn. 2001. Microbes in African Dust Clouds Pose Potential Health Risks. People, Land and Water. 8(4):36.
8. Griffin, D.W., C.A. Kellogg, V.H. Garrison, C. Holmes, and E.A. Shinn. 2002. The Movement of Soil and Sediment in Earth’s Atmosphere: Microbiology and Ecosystem Health. Epidemioecology News. 1(2):1-3
9. Kellogg, C.A., and D.W. Griffin. 2003. African Dust Carries Microbes Across the Ocean: Are They Affecting Human and Ecosystem Health? USGS Open-File Report 03-028
10. Griffin, D.W., C.A. Kellogg, J.T. Lisle, and E.K. Lipp. 2003. Human fecal indicator bacteria and pathogenic viruses in nearshore to offshore reefs in the middle Florida Keys (Long Key, Fl) Florida Keys National Marine Sanctuary Final Permit Report.
11. Lipp, E.K., and D.W. Griffin. 2003. Human enteric viruses in coral mucus of Florida Keys. Report to the Florida Marine Research Institute - Coral Reef Monitoring Program.
12. Katz, B.G., Griffin, D.W., Swarzenski, P.W., Walsh, S.J., and Jelks, H.L., 2003, Florida springs interdisciplinary science study: U.S. Geological Survey Fact Sheet FS-008-03, 4 p.
13. Griffin, D.W., and E.A. Shinn. 2004. Out of Africa: Airborne dust carries microbes to Florida, Southeast. People, Land and Water. 10(4):38
14. Katz, B., J. Brock, R. Edwards, D. Griffin, J. Kindinger, E. Raabe, P. Swarzenski, J. Bales, L. Chasar, M. Darst, T. Grubbs, H. Light, G. Mahon, A. Brooks, K. Dodd, A. Foster, T. Gross, H. Jelks, C. Langtimm, C. McIvor, K. Sulak, S. Walsh, and J. Giller. 2004. An Integrated Science Program for Addressing Relevant Water and Ecology Resource Issues in the Suwannee River Basin and Estuary. USGS White Paper.
15. Lipp, E.K., D.W. Griffin, and J.B. Rose. 2004. Human fecal indicator bacteria and pathogenic viruses in offshore reefs and human recreational risk in nearshore waters of the Florida Keys. Quarter 2 Report to the U.S. Environmental Protection Agency, Marathon, Florida.
16. Lipp, E.K., and D.W. Griffin. 2004. Human Enteric Viruses in Coral Mucus in the Florida Keys. Report to the Florida Marine Research Institute – Coral Reef Monitoring Program.
17. IODP Leg 209 summary. In Kelemen, P.B., Kikawa, E. Miller, D.J., Abe, N. Bach, W. Carlson, R.L., Casey, J.F., Chambers, L.M., Cheadle, M. Cipriani, A., Dick, H.J.B. Faul,, U., Garces, M., Garrido, C., Gee, J.S., Godard, M.M., Graham, D.W., Griffin, D.W., Harvey, J., Ildefonse, B., Iturrino, G.J., Josef, J., Meurer, W.P., Paulick, H., Rosner, M., Schroeder, T., Seyler, M., and Takazawa, E. 2004. Proceedings of the Ocean Drilling Program, Initial reports. 209: College Station TX (Ocean Drilling Program), 1-139.
18. Lipp, E.K., D.W. Griffin, and J.B. Rose. 2004. Human fecal indicator bacteria and pathogenic viruses in offshore reefs and human recreational risk in nearshore waters of the Florida Keys. Quarter 3 Report to the U.S. Environmental Protection Agency, Marathon, Florida.
19. Griffin, D.W. 2004. Florida’s Geology Makes Wastewater Disposal a Potential Threat to Ecosystem Health in the Florida Keys. Sound Waves, Monthly Newsletter, USGS. October issue.
20. Nipper, M., R.S. Carr, V. Garrison, G. Smith, D. Griffin, and J.W. Tunnell Jr. 2005 Effects of globally transported African dust to Caribbean marine ecosystems. Progress report. National Oceanic and Atmospheric Association, National Undersea Research Program.
21. Lipp, E.K., D.W. Griffin, and J.B. Rose. 2005. Human fecal indicator bacteria and pathogenic viruses in offshore reefs and human recreational risk in nearshore waters of the Florida Keys. Period: 11/01/04 – 1/31/05. Report to the U.S. Environmental Protection Agency, Marathon, Florida.
22. Honda, M., H. Sakoh, K. Hayashi, T. Idai, S. Kimura, D. Griffin, F.M. Lutfi, N. Takahashi, F. Kobayashi, T. Noguchi, F. Shibata, M. Moro, Y. Ishikawa, A. Yasuda, T. Shiozaki, J. Hamanaka, Y. Otsubo, K. Nishijima, W. Tokunaga, and R. Ohyama. 2005. RM05-01 (28 February 2005 – 24 March 2005), Preliminary Cruise Report. May 2005, Japan Institute for Marine Earth Science Technology (JAMSTEC).
23. Nipper, M., J.W. Tunnell Jr., R.S. Carr, V.H. Garrison, D. Griffin, G. Smith. 2006. Effects of globally transported African dust to Caribbean marine ecosystems. Final report. NOAA, National Undersea Research Program.
24. Chasar, L., B. Katz, and D. Griffin. 2006. Evaluation of nitrate sources in springs of the Santa Fe River Basin using natural tracers: Geochemical, specific microbiological, and multiple stable isotopic indicators. Project report. Alachua County Environmental Protection Department.
25. Lipp, E.K., D.W. Griffin, J.B. Rose, J.C. Futch, and Y. Masago. 2006. Human fecal indicator bacteria and pathogenic viruses in offshore reefs and human recreational risk in nearshore waters of the Florida Keys. USEPA final report.
26. Plumlee, G.S., W.T. Foreman, D.W. Griffin, J.K. Lovelace, G.P. Meeker, and C.R. Demas. 2007. Characterization of Hurricane Katrina flood sediments and their potential implications for the environment and human health. USGS Circular 1306:245-256
27. Plumlee, G.S., G. Meeker, D. Griffin, W. Foreman, E. Furlong, and C. Demas. 2007. A rationale and process for establishing a formal USGS capability in environmental disaster response and preparedness. USGS Administrative Report. Prepared as part of the USGS Bureau Venture Capital Project: Rapid response environmental and health hazard characterization of solids generated by extreme events.
28. Kelemen, P.B., Kikawa, E. Miller, D.J., Abe, N. Bach, W. Carlson, R.L., Casey, J.F., Chambers, L.M., Cheadle, M. Cipriani, A., Dick, H.J.B. Faul,, U., Garces, M., Garrido, C., Gee, J.S., Godard, M.M., Graham, D.W., Griffin, D.W., Harvey, J., Ildefonse, B., Iturrino, G.J., Josef, J., Meurer, W.P., Paulick, H., Rosner, M., Schroeder, T., Seyler, M., and Takazawa, E. 2007. 2007 Proceedings of the Ocean Drilling Program, Scientific Results. 209: College Station TX (Ocean Drilling Program): processes in a 20-km-thick conductive boundary layer beneath the Mid-Atlantic Ridge, 14o-16oN, 1-33. doi:10.2973/odp.proc.sr.209.001.2007.
29. Lipp, E.K., and D.W. Griffin. 2007. Identification of land-based pollution in South Florida coral reefs: Host specific viruses as conservative markers for human sewage. USEPA progress report.
30. Buxton, H.T., D.W. Griffin, and B.S. Pierce., eds., 2008. Earth science and public health: Second national conference on USGS health-related research, Reston, Virginia, February 27 – March 1, 2007: U.S. Geological Survey, Scientific Investigations Report 2008-5022.
31. Griffin, D.W. 2009. African desert dust in the Amazon Basin: Implications for human and ecosystem health. Proceedings of the 1st International Congress of Environmental Medicine, September 5th – 7th, 2009. UEA, College of Health Sciences. Manaus, Brazil.
32. Griffin, D.W. and D.B. Smith. 2010. A survey of North American soils for selected pathogenic prokaryotes. June quarterly progress report.
33. Katz, B.G., and D.W. Griffin. 2010. Using Multiple Chemical and Microbiological Indicators to Assess Groundwater Pathways and Contaminant Transport. U.S. Geological Survey Fact Sheet. In Press.
34. Griffin, D.W. and D.B. Smith. 2010. A survey of North American soils for selected pathogenic prokaryotes. USEPA October quarterly progress report.
35. Smith, D.J., A.C. Schuerger, D.W. Griffin, R.D. McPeters and P.D. Ward. Life on the edge of space: Characterizing microbial diversity and survival in the stratosphere through high altitude sampling and environmental chamber experiments. Conference Proceedings, Astrobiology Science Conference 2010. http://www.lpi.usra.edu/meetings/abscicon2010/pdf/5006.pdf
36. Griffin, D.W. 2011. The use of epifluorescent microscopy and quantitative polymerase chain reaction to determine the presence/absence and identification of microorganisms associated with domestic and foreign wallboard samples. U.S. Consumer Product Safety Commission. http://www.cpsc.gov/info/drywall/USGSmicrobiological.pdf. Utilized by CPSC for a U.S. Congressional hearing.
37. Griffin, D.W. 2011. Introduction to Bacillus luciferensis. North Pond Expedition 2011, The Adopt-A-Microbe Project v. 4.0. Center for Dark Energy Biosphere Investigations. www.darkenergybiosphere.org/adoptamicrobe/2011/10/introduction-to-bacillus-luciferensis/
38. Griffin, D.W. 2011. Long-range transatlantic transport of microorganisms in clouds of African desert dust: a study of atmospheric microbiology, Chemistry, and the influence of desert dust on surface water microbial ecology about the R/V JOIDES Resolution, IODP Expedition 336, 16 September – 16 November 2011. In Edwards, K.J., Bach, W., Klaus, A., and the Expedition 336 Scientists, Proc. IODP, 336: Tokyo (Integrated Ocean Drilling Program Management International, Inc.) doi:10.2204/iodp.proc.336.110.2012.
39. IODP Expedition 336 Scientists. 2011. Integrated Ocean Drilling Program Expedition 336 Preliminary Report. Mid-Atlantic Ridge microbiology. Initiation of long-term coupled microbiological, geochemical, and hydrological experimentation within the seafloor at North Pond, western flank of the Mid-Atlantic Ridge. 16 September – 16 November 2011. http://publications.iodp.org/preliminary_report/336/
40. Griffin, D.W. 2012. U.S. Geological (USGS)/U.S. Environmental Protection Agency (USEPA) Natural Background Bacteria within U.S. Soils Collaborative Project: Final USEPA report.
41. Lang, J., P. Dustan, E. Peters, R. Dodge, J. Fauth, J. Boyer, P. Gardinali, D. Griffin, V. Kosmynin, B. LaPointe, E. Lipp, M. Miller, V. Paul, E. Shinn, A. Soloviev, and P. Swart. 2013. A Quick Guide to Florida’s Gold Coast Reefs. South Florida Coral Reef Initiative (SEFCRI) Technical Advisory Committee (TAC) outreach document. Published 17 January 2013.
42. Northup, D., D.W. Griffin, and M. Gray. 2013. Cave aeromicrobiology in Carlsbad Caverns National Park. Investigators annual report to the U.S. National Park Service.
43. Bowling, C., D. Griffin, D. Mattorano, E. Silvestri, F. Schaefer and T. Nichols. 2014. USEPA/USGS collection protocol for bacterial pathogens in surface soil. EPA/600/R-14/027.
My Science Topics
Global transport of microorganisms and toxic compounds in clouds of desert dust
One of the most surprising bits of trivia about this field of science is how few scientists are actively involved in research. Long range dispersion of microorganisms, nutrients, and toxic compounds via our atmosphere may impact the health of downwind native ecosystems, crops, livestock, and humans (and there is the benefits of dust transport - many terrestrial and marine plants in downwind environments derive nutrients from dust). At the microbial scale it is not hard to imagine how this mechanism may influence the presence of species and genetic elements in any potential downwind environment no matter the distance.........this is a wide-open and lightly explored field....are you interested?
USGS Microbiology Laboratory - St. Petersburg (USGS-MLSP)
Study types - Viral source tracking - Limitations of the current water quality indicators - Pathogen monitoring in soils, water, and air - Desert dust microbiology - Extreme altitude, lower altitude and cave aeromicrobiology.
1. Standard, reverse transciptase, quantitative and digital polymerase chain reaction assays.
2. Standard and epifluorescent (for bacterial, fungal, viral, and protozoa pathogen ID, microbial direct counts, and cellular studies) microscopy.
3. ZEISS epiflourescent microscope is equipped with a micromanipulator for cell capture.
4. Immunomagnetic separation and other methods for cell separation/capture.
5. High and low volume membrane filtration for both aquatic and atmospheric studies.
6. High volume liquid impingement for atmospheric studies.
7. Particle counter and particle characterization for atmospheric studies.
8. Equipped for indicator and host/phage studies.
9. Cloning and sequence based studies.
10. Experienced and equipped for extraction of nucleic acids from 'problem samples'
11. Laboratory is USDA permitted for the import of foreign soils.
1. Co-PI. Enteroviral analysis of Crystal Springs. Perrier Water. 1999-2000. $59,242.
2. Co-PI. Enteroviral analysis of Crystal Springs. Perrier Water. 2000-2001. $59,242.
3. PI. Rapid Identification of Microbes and Microbial Communities Isolated from Air, Soil and Water using a Biolog 62401A MicroStation System. USGS. 2001-2003. $42,913.
4. Co-PI. Combined Use of Microbiological and Geochemical Techniques to Determine Sources and Fate of Nitrate in Spring Waters. Leon County, Florida. 2003-2004. $100,000.
5. PI. African Dust as a Source of Pathogens to Reef Environments. USGS. 2002-2003. $29,772.
6. Co-PI. U.S. Global Dust Program. USGS. 2003-2006. $2,500,000.
7. Co-PI. Human fecal indicator bacteria and pathogenic viruses in offshore reefs and human recreational risk in nearshore waters of the Florida Keys. USEPA/FKNMS. 2003-2004. $99,923.
8. Co-PI. Delineation of the movement of recharged wastewater from the southeast spray-field through the upper Floridan aquifer, Leon and Wakulla Counties, Florida. City of Tallahassee. 2004-2006. $165,000.
9. Co-PI. Measuring the impact of wastewater on concentrations of nutrients, fecal bacteria, and human enteric viruses in ground and surface water in Dry Tortugas National Park. USGS/NPS. 2004-2006. $60,000.
10. Co-PI. Tracing reclaimed water recharged from the Lake City spray-field into the ground water basin for Ichetucknee Springs. FDEP. 2005. $100,000.
11. Co-PI. South Florida Geographic Initiative, Special Study – Transport of pollutants by ground water – Task 1. USEPA. 2006. $80,000.
12. Co-PI. Identification of land-based pollution in South Florida coral reefs: Host specific viruses as conservative markers of human sewage. USEPA. 2006-2008. $52,490.
13. PI. South Florida Geographic Initiative, Special Study – Identification of sources and signals of land-based pollution emanating from coastal submarine groundwater discharge sites – Task II. USEPA. 2007-2008. $100,000.
14. Co-PI. Chemical and microbiological characterization of the Saint Marks River. NOAA. 2008-2009. $100,000.
15. PI. Human fecal microflora as a source of coral pathogens in the Dry Tortugas National Park: Are coral pathogens invasives or endemic. 2009-2010. USGS/USNPS Park Oriented Biological Support Program. $69,958.
16. PI. Desert dust microbiology and human health. 2009. US Navy, BUMED. $120,000.
17. Co-PI. Microbial water quality affects from lower flows and increased migratory bird densities induced by agricultural and natural resources management decisions in the Central Platte river basin ecosystem. 2009-2011. USGS. $299,900.
18. PI. Microbial and chemical analyses of Asian desert dust impacting air quality in the NW continental United States. 2010. USGS. $29,000.
19. PI. A continental U.S. soil survey for background concentrations of Bacillus anthracis, Yersinia pestis and Francisella tularensis. 2010-2011. USEPA. $410,000.
20. PI. Mimicking spherical dust as a drug delivery system. 2010. DARPA. $40,000.
21. Co-PI. Climate-change based predictive modeling of rising Vibrio illness in the U.S. 2011-2012. US Centers for Disease Control. $97,205.
22. PI. Microbial analyses of Chinese wallboard samples. 2011. U.S. Consumer Protection Safety Commission. $40,000.
23. PI. Screening test samples for biological warfare agent surrogates. 2011. USEPA. $30,000.
24. PI. Pathogenic bacteria in North American soils: A joint USGS – USEPA study. 2012-2013. USEPA. $75,000.
25. PI. Pathogenic bacteria in North American soils: A joint USGS – USEPA study. 2013-2014. USEPA. $100,000.
26. PI. An analysis of water samples using Quanti-trays. 2014-2015. University of Oklahoma. $28,000.
27. PI. Atmospheric detection of Coccidioides immitis. 2014. US Centers for Disease Control. $20,000.
28. PI. Pathogenic bacteria in North American soils: A joint USGS – USEPA study. 2014-2015. USGS Contaminate Biology Program. $104,555.
29. Co-I. The U.S. Geological Survey’s Sediment-bound Contaminant Resiliency and Response Strategy: A Tiered Multi-metric Approach to Environmental Health and Hazards in the Northeastern USA. 2015-2016. USGS. $2,000,000.
30. PI. Pathogenic bacteria in North American soils: A joint USGS – USEPA study. 2014-2015. USEPA. $75,000.
31. PI. Detection of toxins and mutagens in marine sediments of SE Florida. 2014-2015. FLDEP. $13,864.
32. PI. Microbial method development and geographic information systems project. 2014-2015. USEPA. $25,000.
600 4Th Street South
St. Petersburg, FL 33701
727-502-8001 - Fax
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